Computer-aided subsite mapping of α-amylases

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Subsite mapping of enzymes. Application of the depolymerase computer model to two alpha-amylases.

In the preceding paper (Allen and Thoma, 1976) we developed a depolymerase computer model, which uses a minimization routine to establish a subsite map for a depolymerase. In the present paper we show how the model is applied to experimental data for two alpha-amylases. Michaelis parameters and bond-cleavage frequencies for substrates of chain lengths up to twelve glucosyl units have been repor...

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ژورنال

عنوان ژورنال: Carbohydrate Research

سال: 2011

ISSN: 0008-6215

DOI: 10.1016/j.carres.2010.12.002